The DETERMINATION THE FREQUENCY OF MYELOID LYMPHOBLASTIC LEUKEMIC GENE REARRANGEMENTS IN PEDIATRIC ACUTE LYMPHOBLASTIC LEUKEMIA
Objectives: To determine the prevalence of gene rearrangements of myeloid lymphoblastic leukemia in pediatric acute lymphoblastic leukemia.
Material & Methods: This Descriptive, Cross-sectional study was conducted at Department of Hematology, Combined Military Hospital Multan and Shaukat Khanum Memorial Cancer hospital and Research Centre, Lahore from 19th March 2019 to 18th September 2019. Total 145 patients of acute lymphoblastic leukemia with 1-12 years of age and all genders were included. Patients taking conventional chemotherapy or on any tyrosine kinase inhibitor were excluded. Bone marrow samples were sent to Shaukat Khanum Memorial Cancer hospital, Lahore for fluorescent hybridization (FISH). Culturing of bone marrow specimens was done at 37°C, for 48 and 72 hours in Roswell Park Memorial Institute and 10% fetal bovine serum without any mitogen (unstimulated) to the medium. Colcemid, 0.02 μg/ml was put into the cultures 30 min earlier before harvesting. Potassium Chloride, 0.075 M was used to give hypotonic treatment to the cells. After this the cells were fixed with CH3OH (methanol) and CH3COOH (acetic acid) in proportion of 3:1. Finally, the slides of the cells were prepared and MLL gene rearrangements were noted.
Results: Mean age was 6.03 ± 2.23 years. Majority of the patients 94 (64.83%) were between 1 to 6 years of age. Out of 145 patients, 90 (62.07%) were females and 55 (37.93%) were male with female to male ratio 1.6:1. The frequency of myeloid lymphoblastic leukemia gene rearrangements in pediatric ALL was found in 54 (37.24%) patients.
Conclusion: We concluded that a very high MLL rearrangements frequency in pediatric ALL was found in our study.
Key Words: Pediatric, Acute lymphoblastic leukemia, MLL rearrangements.