IMMUNOHISTOCHEMICAL LOCALIZATION OF KERATIN IN 100 CASES OF SQUAMOUS CELL CARCINOMA

Authors

  • Gull -e- Atif
  • A H Nagi
  • Salma Naz

Abstract

Introduction: Squamous cell carcinomas range from well differentiated to poorly differentiated and these vary in prognosis. Immonohistochemical marker study was done to assess the extent of differentiation.

Objectives: To detect the presence of keratin in apparently non-keratinizing poorly differentiated squamous cell carcinomas by immunoperoxidase staining.This is important because keratinizing tumours are less radiosensitive and non-keratinizing are more radio responsive.

Study design: A cross sectional and descriptive study.

Setting and duration of study: King Edward Medical College, Post Graduate Medical Institute,Lahore. Duration of the study was one year .

Materials and methods:A total of 100 patients suffering from squamous cell carcinomas were included in the study.Both H and E and immunoperoxidase stainings were performed. The results of both types of staining were compared for each case.

Results: 4 groups were identified.49 cases showed obvious keratinization on both H and E and immunoperoxidase staining.20  cases had doubtful keratinization on H and E but showed more obvious keratinization on immunoperoxidase staining. 10 cases were non-keratinizing on H and E staining but revealed keratin on immunoperoxidase analysis.21 cases were non-keratinizing on H and E as well as on immunoperoxidase analysis.

Conclusion:Cytokeratin can be used as a tumour marker for squamous cell carcinoma. The immunohistochemical technique can help us in revising and modifying our H and E impression of a squamous cell carcinoma.

Key words: Squamous Cell Carcinoma, Cytokeratin, Immunoperoxidase.

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Published

03-08-2016

How to Cite

1.
Atif G- e-, Nagi AH, Naz S. IMMUNOHISTOCHEMICAL LOCALIZATION OF KERATIN IN 100 CASES OF SQUAMOUS CELL CARCINOMA. Pak J Pathol [Internet]. 2016 Aug. 3 [cited 2024 Mar. 29];20(3). Available from: https://pakjpath.com/index.php/Pak-J-Pathol/article/view/245

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