IMMUNOHISTOCHEMICAL LOCALIZATION OF KERATIN IN 100 CASES OF SQUAMOUS CELL CARCINOMA
Abstract
Introduction: Squamous cell carcinomas range from well differentiated to poorly differentiated and these vary in prognosis. Immonohistochemical marker study was done to assess the extent of differentiation.
Objectives: To detect the presence of keratin in apparently non-keratinizing poorly differentiated squamous cell carcinomas by immunoperoxidase staining.This is important because keratinizing tumours are less radiosensitive and non-keratinizing are more radio responsive.
Study design: A cross sectional and descriptive study.
Setting and duration of study: King Edward Medical College, Post Graduate Medical Institute,Lahore. Duration of the study was one year .
Materials and methods:A total of 100 patients suffering from squamous cell carcinomas were included in the study.Both H and E and immunoperoxidase stainings were performed. The results of both types of staining were compared for each case.
Results:Â 4 groups were identified.49 cases showed obvious keratinization on both H and E and immunoperoxidase staining.20Â cases had doubtful keratinization on H and E but showed more obvious keratinization on immunoperoxidase staining. 10 cases were non-keratinizing on H and E staining but revealed keratin on immunoperoxidase analysis.21 cases were non-keratinizing on H and E as well as on immunoperoxidase analysis.
Conclusion:Cytokeratin can be used as a tumour marker for squamous cell carcinoma. The immunohistochemical technique can help us in revising and modifying our H and E impression of a squamous cell carcinoma.
Key words: Squamous Cell Carcinoma, Cytokeratin, Immunoperoxidase.Downloads
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Copyright (c) 2016 Gull -e- Atif, A H Nagi, Salma Naz
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