BACTERIAL PROFILE AND ANTIBIOGRAM OF MICROORGANISMS ISOLATED FROM DIFFERENT BODY SITE INFECTIONS AMONG PATIENTS OF A PRIVATE HOSPITAL, KARACHI

Authors

  • Shaista Bakhat Bahria University Medical and Dental College, Karachi Pakistan
  • Saman Nadeem National Medical Centre, Karachi Pakistan
  • Yasmeen Taj Bahria University Medical and Dental College, Karachi Pakistan
  • Beenish Hussain Bahria University Medical and Dental College, Karachi Pakistan
  • Ashfaq Hussain Combined Military Hospital, Mardan Pakistan
  • Danish Shakeel National Medical Centre, Karachi Pakistan

DOI:

https://doi.org/10.55629/pakjpathol.v34i1.729

Abstract

Objective:It is crucial to keep an eye on current patterns of multi-drug resistance and extensively drug resistance at local level to facilitate physicians in making justified decision regarding empirical therapy during this era of rising superbugs.

Material and Methods: This antibiogram was made up according to CLSI (Clinical Laboratory Standard Institute) M100 guidelines. Samples were received for a period of 2-years from June 2019-June 2021 at National Medical Center Karachi. These samples were urine, blood, pus, cerebrospinal fluid (CSF), central venous pressure (CVP) tip, sputum and tracheal aspirates.

Results: Out of 10564 samples received, 4582 were positive for growth of microorganisms. Sample distribution patterns according to the frequency of positive growth of isolates were predominantly urine (58%) and blood (17%) followed by pus (8%) and pus swab (6%) 2% fluid and 2% tracheal secretions, and 7% others like tips growth.  Blood cultures were mainly received from Intensive care unit (50%) followed by out-patients departments (34%). The pattern of resistance observed in Escherichia coli (E. coli) revealed ampicillin as the most resistant among all antibiotics (93%) followed by cefixime (77%). Most sensitive antibiotic was amikacin (99%).  The Staphylococcus aureus isolates were highly susceptible to vancomycin and linezolid (100%) followed by gentamicin (89%) and chloramphenicol (85%). Vancomycin resistance was observed in Enterococcus faecium (8%).

Conclusion: Antimicrobial resistance among gram negative and gram-positive bacteria is increasing. This has left us with fewer therapeutic options which in turn highlights the issue of antimicrobial resistance being a global health concern. This emphasizes the significance of surveillance of antimicrobial patterns, development of antibiograms and implementation of antibiotic stewardship programs by judicious use of antibiotics for prevention of further spread of antimicrobial resistance.   

Key Words: Antibiogram, Antimicrobial resistance, Multidrug resistance, Extensively drug resistance

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Published

31-03-2023

How to Cite

1.
Bakhat S, Nadeem S, Taj Y, Hussain B, Hussain A, Danish Shakeel. BACTERIAL PROFILE AND ANTIBIOGRAM OF MICROORGANISMS ISOLATED FROM DIFFERENT BODY SITE INFECTIONS AMONG PATIENTS OF A PRIVATE HOSPITAL, KARACHI. Pak J Pathol [Internet]. 2023 Mar. 31 [cited 2024 Dec. 22];34(1):11-6. Available from: https://pakjpath.com/index.php/Pak-J-Pathol/article/view/729

Issue

Vol. 34 No. 1 (2023): Pakistan Journal of Pathology

Section

Articles

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Copyright (c) 2023 Shaista Bakhat, Saman Nadeem, Yasmeen Taj, Beenish Hussain, Ashfaq Hussain, Danish Shakeel

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