IMMUNOHISTOCHEMICAL EXPRESSION LEVELS OF CYCLIN E1 IN THREE HISTOLOGICAL GRADES OF ORAL SQUAMOUS CELL CARCINOMA
Objective: The objective of this study is to assess and compare the immunohistochemical expression levels of Cyclin E1 in three histological grades of oral squamous cell carcinoma.
Material and Methods: A descriptive cross-sectional study was designed. Sample size was 90 and categorised into three groups as i.e., well, moderate and poorly differentiated oral squamous cell carcinoma (OSCC) with 30 cases in each group. Specimens were collected from Histopathology Laboratories at Pakistan Institute of Medical Sciences (PIMS) and Foundation University Dental College and Hospital (FUDC&H) Islamabad, to evaluate the expression levels of Cyclin E1 (CCNE1) with the help of immunohistochemical (IHC) staining. Statistical analysis was performed using the statistical analysis software, SPSS version 25.
Results: In OSCC cases, average age of patients was 61.1±10.2 years. Over all age range of patients was 40-79 years (min – max) which was statistically significant (p=0.02). Male to female ratio was 1.1:1 (p=0.003). The most common site of development of OSCC was Buccal mucosa (p= < 0.001). According to immunoreactive scoring system (IRS) value of CCNE1 expression levels was low in 42.2% of cases and high in 57.7% of cases. The high final score of 6 and 9 was found in 80% cases of poorly differentiated OSCC cases and the results were found statistically significant (p<0.001)
Conclusion: It is concluded that increased expression levels of Cyclin E1 significantly correlated with lack of differentiation in OSCC.
Key Words: Cyclin E1, Immunohistochemistry, Oral squamous cell carcinoma.
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