CLINICOPATHOLOGICAL AND IMMNNOHISTOCHEMICAL PROFILE OF LYMPHOMA OF HEAD AND NECK REGION

  • Uzma Jabbar Khan University College of Dentistry, University of Lahore, Lahore Pakistan
  • Wajiha Alamgir University College of Dentistry, University of Lahore, Lahore Pakistan
  • Aroosa Ashraf University College of Dentistry, University of Lahore, Lahore Pakistan
  • Adeel Haider University College of Dentistry, University of Lahore, Lahore Pakistan
  • Salman Rashid Avicenna Medical and Dental College, Lahore Pakistan
  • Faheem Abrar 3Punjab Health Care Commission, Lahore Pakistan

Abstract

Objective: The study was intended to analyze the frequency distribution and relative sites of occurrence of extra nodal lymphoma in head and neck region. Moreover, it was also focused to set down the histological types and subtypes of the diagnosed lymphoma through various immunohistochemical panels. The objective of the study is to evaluate the frequency of co-expression of c-Myc and BCL-2 in Diffuse large B-cell lymphoma.

Material and Methods: This cross-sectional observational study was conducted in the department of Oral and Maxillofacial Pathology of University Dental Hospital, University of Lahore, Lahore. Total 39 proven cases of head and neck lymphoma from period of June 2018 to July 2021 were included. Inclusion criteria consist of lymphoma of head and neck region in age group 25years to 100 years. Exclusion criteria comprised of lymphoma in age less than 25 years and lymphoma of CNS. The immunohistochemical panel used included the antibodies against LCA, CD3, CD10, CD15, CD20, CD23, CD40, CD43, CK, Cyclin D1, Bcl2, GCDFP-15, Kappa and Lambda. SPSS version 25 was used to analyze the data. Pearson chi square was applied and p < 0.05 was taken as significant.

Results: Out of 39 cases, 21 (54%) were males and 18 (46%) were females. The mean age was 57.89 +16.43 years. Out of the total, 38 cases were diagnosed as Non-Hodgkin lymphoma (NHL) and only one turned out to be Classical Hodgkin lymphoma of mixed cellularity type. Among NHL diffuse large B cell Lymphoma (DLBCL) was greatest in number (n=29 ,76.31%), followed by 6 cases of marginal zone (15.3%) and one case each of small lymphocytic lymphoma, follicular lymphoma and unspecified peripheral T cell lymphoma. The most common extra nodal site involved was tonsil n=18 (46.5%), followed by parotid gland n=6 (15.38%), palate n=3 (7.69%), alveolar ridge (n=2 ,5.12%), tongue n=2 (2.15%), submandibular region n=2 (5.12%), buccal mucosa n=2 (5.12%), upper jaw n=1 (2.56%), oropharynx n=1 (2.56%), gingiva n=1 (2.56%) and lip n=1 (2.56%)

Conclusion: Non-Hodgkin lymphoma comprise of 97.4% of total cases where as Hodgkin lymphoma only 2.5%. Most common site involved was tonsil with greatest frequency of diffuse large B cell lymphoma.

Key Words: Hodgkin Lymphoma, Non-Hodgkin Lymphoma, Immunohistochemistry, Diffuse Large B cell lymphoma.

Published
2021-12-31