EFFICACY OF CEFTAROLINE AGAINST METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS: A CROSS-SECTIONAL STUDY
Objective: To evaluate in vitro efficacy of Ceftaroline against Methicillin resistant Staphylococcus aureus (MRSA) isolated in our setup.
Material and Methods: This descriptive cross-sectional study was carried out at Department of Microbiology, Fauji Foundation Hospital, Rawalpindi from November 2018 to April 2019. Clinical samples from respiratory tract, blood, pus, urine and various catheter tips were inoculated on Blood, MacConkey’s agar and Incubated at 37ᵒC for 18 to 24h. Staphylococcus aureus was identified by standard microbiological procedures. Methicillin resistance of isolated Staphylococci was detected by agar disk diffusion method (Kirby-Bauer) according to CLSI guideline. The Minimum Inhibitory Concentrations (MICs) were determined for ceftaroline and vancomycin by using the Epsilon-test (E-test) method. S.aureus ATCC29213 was used as control strain for MIC detection.
Results: Total of 55 samples were included according to the inclusion criteria of the study. Frequencies and percentages of sensitive, intermediate and resistant organisms according to MIC of ceftaroline against MRSA isolates were 52(94.5%), 3(5.4%) and 0 respectively. MIC when calculated according to CLSI guidelines, MIC50 and MIC90 for ceftaroline against MRSA isolates was 0.75 and 1µg/ml respectively.
Conclusion: We can safely conclude that in vitro ceftaroline is more effective than vancomycin against MRSA.
Key Words: Ceftaroline, Methicillin-resistant Staphylococcus aureus, Minimum inhibitory concentration.