DIAGNOSTIC ACCURACY OF IMMUNOFLUORESCENCE IN GLOMERULONEPHRITIS: A SINGLE CENTER EXPERIENCE OF 150 CASES
Objective: The objective of this study was to investigate the diagnostic role of immunofluorescence (IF) in accurate diagnosis of various glomerulonephritis (GN) in Pakistani population.
Material and Methods: Cross sectional study of one-year duration from January, 2019 to December, 2019 was conducted at histopathology department, Chughtai Institute of Pathology, Lahore, Pakistan. A total of 150 cases of GN were included in this study in which light microscopy (LM) and IF findings were reviewed. All clinical and biochemical data was recorded on a proforma for each case.
Statistical methods use to analyze this study included mean ± SD, percentages, ratio, sensitivity, specificity, positive predictive value and negative predictive value.
Results: Of the total cases, 79 (52.7%) were males and 71(47.3%) were females. Mean age of the patients was 24.29±15.07 years. IF helped in changing the diagnosis of LM in 28 cases (18.67%). In this study, the most common pattern of GN diagnosed was membranous GN (30.6%) followed by focal segmental glomerulosclerosis (28%), lupus nephritis (11.3%) and membranoproliferative GN (7.3%). The sensitivity of IF was 96.5% and specificity was 94.4%.
Conclusion: Our study reinforces the fact that IF in conjunction with LM plays crucial role in the diagnosis of GN along with relevant clinical and biochemical data.
Key Words: Light Microscopy, Immunofluorescence, Electron microscopy, Glomerulonephritis, Focal Segmental Glomerulosclerosis.