HISTOPATHOLOGICAL EVALUATION OF PROTECTIVE EFFECT OF HONEY AGAINST ACETAMINOPHEN-INDUCED HEPATOTOXICITY IN WISTAR ALBINO RATS
Objective: The morphological evaluation of the protective effects of honey against acetaminophen induced hepatotoxicity and to estimate the effects of honey on serum hepatic biomarkers in controls and honey treated Wistar albino rats.
Material and Methods: This experimental study was carried out for 6 months at animal house; Isra University, Hyderabad and Department of Animal Husbandry and Veterinary Sciences Sindh Agriculture University Tando Jam. A total of 60 albino Wistar healthy rats from either sex, 200-300g weight were divided into 05 groups, each comprising of 12 rats (the groups were from A to E namely Negative control group, Positive control group, Pre-treatment group, treatment group and Post-treatment group respectively, in whom doses of paracetamol and honey were given at different time). At the end of 8th week, rat’s blood samples were drawn through cardiac puncture for biochemical analysis and liver tissue samples were preserved in 10% formalin, followed by processing for histopathological examination.
Results: The present study showed a significant rise in serum bilirubin, ALT and ALP levels in the positive control (Group B) as compared to the negative control group. However, administration of honey significantly decreased the serum bilirubin levels in groups C, D and E. Liver sections from the acetaminophen treated group revealed wide areas of centrilobular coagulative necrosis, inflammatory cell infiltrates and severe congestion and dilatation in both central and portal vein in 83.33 %, 100 % and 100 % of the cases respectively. Honey treated groups given before, after and simultaneously showed improvement with restoration of histologic changes with statistically significant p-value (0.001).
Conclusion: The results of the present study have demonstrated satisfactory protection by honey against acetaminophen-induced toxicity in liver of rats. It recovers the normal cellular structure along with improvement in the biochemical alterations
Key Words: Acetaminophen, Hepatotoxicity, Serum bilirubin.