COMPARISON OF GLUT1 EXPRESSION IN ORAL SQUAMOUS CELL CARCINOMA WITH NORMAL MUCOSA
Objective: In cancerous cells transport of glucose across the cell membrane is the first rate-limiting step, facilitated by a family of 14 GLUTs. Amongst them GLUT1 is the most important member. The aim of this study was to assess and compare GLUT1 status of oral squamous cell carcinoma (OSCC) patients with normal controls and correlate its expression with grade and stage of the tumor.
Material & Methods: This prospective cross-sectional study consisted of 60 subjects of which 30 were OSCC and 30 were healthy controls. Sampling was done using consecutive convenient sampling from June 2015 to February 2016. Grading and staging of all 30 cases of OSCC was carried out. Immunohistochemical technique was used for analyzing GLUT1 expression in OSCC cases and 30 normal controls. The comparison of scores was performed with Fisher’s Exact test.
Results: Over expression of GLUT1 was detected in 17 (56.67 %) cases of OSCC as compared to normal mucosa with expression in basal layer only (p=0.016). Statistically insignificant results (p=0.571) were obtained with histopathological grades while significant GLUT1 expression (p=0.036) was found with respect to different clinical stages of the tumor.
Conclusion: A statistically significant relationship of GLUT1 expression was observed in OSCC cases which expressed more strongly with advanced clinical stages. However, no significant results could be obtained when GLUT1 expression was compared with grade of the tumor.
Key Words: GLUT1, Oral squamous cell carcinoma, Immunohistochemical stain.