FREQUENCY, DISTRIBUTION AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF BACTERIAL ISOLATES FROM BLOOD CULTURE IN A TERTIARY CARE HOSPITAL
Objective: Blood stream infections remain a serious problem which can lead to morbidity and mortality. It needs immediate and prompt treatment by antibiotics and empirical antibiotic therapy can benefit the patient. This study was done to determine the pattern of bacterial isolates yielded from blood culture specimens and the antibiogram of these isolates to help in providing guidelines for initiation of an appropriate antibiotic therapy for septic patients.
Material and Methods: This descriptive cross-sectional study was conducted at microbiology department, Lahore General Hospital from January to December 2018. Total 2379 blood culture bottles were received and frequency of different bacterial isolates and their antimicrobial susceptibility pattern was determined according to CLSI guidelines.
Results: Total 267/2379 blood culture specimens showed positive growth. It represents 11.22 %. Amongst these Gram-positive cocci were predominant microorganisms (112/267). It was followed by non-fermenters (102/267) and Enterobacteriaceae (52/267). Only 1 sample showed growth of Candida species. Out of total 112 Gram positive cocci, Staphylococcus aureus and coagulase negative Staphylococcus species were 56.25 % and 41.96 % respectively. MRSA accounted for 58.7 %. Amongst enterobacteriaceae 55.76 % were E.coli and Klebsiella species were 34.6 %. Among 102 non-fermenters, 68.6 % were Acinetobacter species and 31.37 % were Pseudomonas species. Overall, the most predominant microorganism isolated was Acinetobacter species (26.2 %). The members of Enterobacteriaceae showed 13.4 % and 15.3 % susceptibility to 3rd and 4th generation cephalosporins. The carbapenem were found most sensitive i.e. 75%.
Conclusion: The results of this study will help to determine the etiology of sepsis and their susceptibility and resistance pattern. This will pave the way for formulating a local antibiotic policy for blood stream infections.
Key Words: Antimicrobial susceptibility, Bacterial isolates, Blood culture.