AN INEXPENSIVE METHOD OF PREPARING EDTA DISKS FOR THE DETECTION OF METALO-BETA-LACTAMASES IN UROPATHOGENIC E COLI
Objective: The aim of this study was to determine the frequency of Metalo-beta lactamases in uropathogenic Escherichia coli and to check the efficiency of in house prepared EDTA disks against them.
Study Design: Descriptive and Quasi experimental.
Place and duration of study: The Microbiology Department, Combined Military Hospital, and Institute of Dentistry Lahore, Pakistan from February 2016 to August 2016.
Material and Methods: Uropathogenic E coli were isolated in 83 urine specimens, 12 isolates had shown resistance to Carbapenems (Imipenem and Meropenem), by Kirby-Bauer disk diffusion method. These isolates were further subjected to Modified Hodge Test as recommended by clinical laboratory standard institute. Isolates which were resistant to carbapenem discs one or both with positive MHT were further subjected to Double disk synergy test and combine disk test using in house prepared EDTA disks 750μg for the detection of Metalo-beta lactamases.
Result: Frequency of Carbapenemases in uropathogenic E. coli was 13.25 % with a frequency of Metalo-b- lactamases to be 9.64%. Double disk synergy test and combine disk test both prove highly sensitive (100%) for the detection of Metalo-beta lactamases.
Conclusion: Double disk synergy test and combine disk test, using in house prepared EDTA disks is an inexpensive technique and improves reporting of Metalo-beta lactamases.
Key Words: Modified Hodge test, Double disk synergy test, Carbapenemases.