PARAPHENYLENE DIAMINE INDUCED HISTOMORPHOLOGICAL CHANGES IN RAT OVARY

Authors

  • Sana Malik Army Medical College (National University of Medical Sciences), Rawalpindi, Pakistan
  • Khadija Qamar Army Medical College (National University of Medical Sciences), Rawalpindi, Pakistan
  • Sabah Rehman Army Medical College (National University of Medical Sciences), Rawalpindi, Pakistan

Abstract

Objective: To evaluate histomorphological changes in ovary of Sprague Dawley rat induced by topically applied paraphenylene diamine (PPD).

Study Design: Laboratory based randomized control trial.

Place and Duration: The study was carried out in the Department of Anatomy, Army Medical College, Rawalpindi in collaboration with National Institute of Health, Islamabad from November 2015 to November 2016.

Material and Methods: Thirty adult Sprague Dawley female rats, weighing 200-300grams, were used and divided into 3 groups with 10 rats in each group. Group A served as control group and animals were applied with distilled water on dorsal surface clipped free of hair. Group B and C were applied with 1mg and 3mg per kg body weight of paraphenylene diamine dissolved in distilled water on dorsal surface clipped free of hair. The solution was applied daily for 30 minutes per day for 60 days. All animals were sacrificed on 60th day of study project. Right ovary of each rat was removed, fixed in 10% formalin, processed, sectioned and stained with H&E. SPSS 21 was used for data analysis.

Results: It was observed on microscopic examination that topically applied paraphenylene diamine solution effects histology of rat ovary by formation of cystic follicles, appearance of interstitial vacuoles and atresia of large follicles.

Conclusion: It was concluded from results that topically applied paraphenylene diamine solution induces histomorphological changes in ovary of rat with 3mg/kg dose inducing statistically significant changes than 1mg/kg dose.

Keywords: Paraphenylene diamine (PPD), Ovary, Sprague Dawley rats, Topical application.

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Published

29-12-2016

How to Cite

1.
Malik S, Qamar K, Rehman S. PARAPHENYLENE DIAMINE INDUCED HISTOMORPHOLOGICAL CHANGES IN RAT OVARY. Pak J Pathol [Internet]. 2016 Dec. 29 [cited 2024 Dec. 19];27(3). Available from: https://pakjpath.com/index.php/Pak-J-Pathol/article/view/363

Issue

Vol. 27 No. 3 (2016)

Section

Articles

License

Copyright (c) 2016 Sana Malik, Khadija Qamar, Sabah Rehman

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